Immunohistology and digital image analysis

DIAPath (Digital Image Analysis in Pathology) is a platform from the CMMI for the validation of tissue-based biomarkers using special staining, standardized immunohistochemistry (IHC) and multiplexed immunofluorescence (mIF), whole slide imaging and image analysis (with artificial intelligence) for objective quantitative analysis.

The overall goal is to extract information useful for understanding disease processes and response to therapy, and to characterize biomarkers useful for diagnostic, prognostic and therapeutic purposes.

The GIGA Immunohistology platform provides the materials, techniques, and expertise required to conduct consistent and reliable histological studies. The facility is equipped for paraffin processing and embedding of both fixed and frozen samples, as well as sectioning of paraffin-embedded or frozen tissues. It can also produce thin-layer cell preparations from cell suspensions upon request and offers both tinctorial and immunohistochemical staining services.

IHC/IF/ISH research platform

• Dako Autostainer Link 48
• Roche Discovery ULTRA

Automated slide stainer

• Leica autostainer XL

Fluorescence whole slide scanners 

• Zeiss Axioscan 7 (Slide scanner, 100 slides magazine, epifluorescence (7 colors), brightfield, phase contrast and polarization microscope).

Brightfield whole slide scanner

• Hamamatsu Nanozoomer S360
• Hamamatsu Nanozoomer S60V2

Image processing and analysis

• Visiopharm, QuPath, ZEN blue